Figure 4. Functional Characterization of Cerebral Organoids.

A, Overlap of upregulated differentially expressed genes (DEGs) with genes expressed in excitatory and inhibitory neurons. B, Overlap of downregulated DEGs with genes expressed in excitatory and inhibitory neurons. C, Quantification of raster plot data collected before and after stimulus. D, Quantification of raster plot data collected with and without 30mM potassium chloride (KCl). Data collected from 4 control and 4 schizophrenia cerebral organoids, with 2 technical replicates in each condition. Values are shown as mean (SD). The data have a normal distribution confirmed via Kolmogorov-Smirnov. Unpaired t test with Welch correction: control stimulus vs schizophrenia stimulus, R², 0.7985; 95% CI, −51.40 to −39.72; P < .001. Control KCl vs schizophrenia KCl, R², 0.8430; 95% CI, −29.83 to −14.67; P < .001.