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SEC-UV of myoglobin using different eluents varying in nature of salt, ionic strength, and pH. (A) Typical chromatograms obtained with eluents of low and high ionic strength, all at pH 7.5. (B) Plots representing the Kd observed for myoglobin versus the eluent ionic strength using the indicated salt. Eluent pH: 5.9 (black), 6.9 (red), and 7.5 (blue). The connecting lines between the points highlight trends and are not obtained by fitting.
