Figure 2.
SEC-UV of the protein test mixture containing thyroglobulin (1), γ-globulin (2), ovalbumin (3), myoglobin (4), and vitamin B12 (5) using eluents consisting of solutions of (a) sodium phosphate, pH 6.9, (b) ammonium acetate, pH 6.9, (c) ammonium formate, pH 7.5, and (d) ammonium bicarbonate, pH 7.5 at concentrations of (A) 0.01 M and (B) 0.2 M.