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SEC-ESI-MS of the protein test mixture using an eluent of (A) 0.01 M ammonium acetate and (B) 0.2 M ammonium acetate (both pH 6.9). Average mass spectra of the myoglobin peaks are provided. Peaks: thyroglobulin (1), γ-globulin (2), ovalbumin (3), myoglobin (4), and vitamin B12 (5).
