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. 2020 Mar 19;10:5061. doi: 10.1038/s41598-020-60810-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of selected altered transcripts in the mucosae of segments of the internal genital tract of the sows in P1-AI group; Endocervix (EndoCvx: (A)), Endometrium (DisEndom: (B), ProEndom: (C)), Utero-tubal Junction (UTJ: (D)), Isthmus (Isth: (E)), Ampulla (Amp: (F)) and Infundibulum (Inf: (G)). The analysis of overrepresented functional categories was performed using the Cytoscape v3.0.0 application ClueGo v2.0.3. Terms are functionally grouped based on shared genes (kappa score) and are shown in different colors. The size of the nodes indicates the degree of significance, where the biggest nodes correspond to highest significance. The following ClueGo parameters were used: GO tree levels 3–6 (first level = 0); minimum number of genes 1; minimum percentage of genes 8, 5P-value correction, Benjamini-Hochberg, terms with P < 0.05, GO term connection restriction (kappa score) 0.4; GO term grouping, GO term fusion; initial group size of 3. The resulting network was modified; that is, some redundant and non-informative terms were deleted and the network manually rearranged.