Table 3.
Biological function of differentially expressed genes (Up & Down regulated) in sperm-free groups among the entire female genital tract of the sow.
| Genes | Tissue | Group | Expression | Biological role |
|---|---|---|---|---|
| GAPDH | Inf | SP-P1 | DOWN | Inition of apoptosis |
| ATM | DistEndom | SP-P1; SP-Ejac | UP | Phosphorylation of key proteins involved in DNA repair and apoptosis |
| Isth | SP-P1 | UP | ||
| IFN-alpha9 | ProxEndom | SP-P1 | DOWN | Activation of immune system |
| ProxEndom | SP-Ejac | UP | ||
| PRLR | ProxEndom | SP-P1; SP-Ejac | DOWN | Prosurvival factor for spermatozoa by inhibiting sperm capacitation through suppression of SRC kinase activation and stimulation of AKT |
| BCL6 | DistEndom | SP-Ejac | DOWN | Modulation of IL-4 and lead the differentiation of naive helper T-cells |
| CDKN2D | DistEndom | SP-Ejac | DOWN | Prevents the activation of CDK kinases |
| G6PC2 | DistEndom | SP-Ejac | DOWN | Catalyzes the final steps of gluconeogenic and glycogenolytic pathways |
| KRAS | DistEndom | SP-Ejac | UP | Controls cell proliferation |
| PRKAB1 | DistEndom | SP-Ejac | UP | Activated protein kinase subunit beta-1 |
| ANAPC13 | DistEndom | SP-Ejac | UP | Controls the G1 phase of the cell cycle |
| HDAC2 | DistEndom | SP-Ejac | UP | Involved in cell cycle progression |
| COL5A3 | ProxEndom | SP-Ejac | DOWN | Collagen alpha-3(V) chain, of the low abundance fibrillar collagens |
| CSF3 | ProxEndom | SP-Ejac | DOWN | Act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages |
| TSC2 | ProxEndom | SP-Ejac | DOWN | Stimulate GTPases. Interacts with Hsp70 and Hsp90 |
| CHST7 | EndoCvx, Dist endom | SP-Ejac | DOWN | Important determinant of sulfated GAGs expression and the associated function of chondroitin sulfates as regulators of many biologic processes |
| CHST3 | Endo cvx | SP-Ejac | DOWN | Related with GAGs as well as CHST7 |
| CHST12 | DistEndom | SP-Ejac | DOWN | Related with GAGs as well as CHST7 |
| DSE | EndoCvx | SP-Ejac | DOWN | Induce T lymphocytes |
| E2F3 | DistEndom | SP-Ejac | DOWN | Transcription factor, control cycle |
| STAG2 | DistEndom | SP-Ejac | UP | Part of the cohesion comples to hold the chromatid togheter |
| FGFR1 | ProxEndom | SP-Ejac | DOWN | Indirect activation of the gamma isoforms of phospholipase C (PLCγ) |
| PPP2R5D | ProxEndom | SP-Ejac | UP | Negative control of cell growth and division |
Sperm-free seminal plasma of from P1-fraction P1 (SP-P1) or of the whole ejaculate (SP-Ejac). All treatments were compared to Control (AI with 50 mL of BTS).