Table 5.
Most significant differently expressed biological pathways (P < 0.05) examined with KEGG database in artificial insemination of P1 fraction (P1-AI) group in comparison with the Control group.
| PATHWAY ID | PATHWAY NAME | ENRICHMENT SCORE | P-VALUE | GENES ALTERED% | GENE LIST | |
|---|---|---|---|---|---|---|
| EndoCvx | ||||||
| 1 | KEGG_103 | Steroid biosynthesis | 6.1 | 0.002 | 12.2 | SQLE, MSMO1, CYP51 |
| 2 | KEGG_110 | N-glycans biosynthesis | 4 | 0.01 | 10.8 | ALG8, B4GALT3, DPM2, UT8 |
| 3 | KEGG_8 | Glycosaminoglycan biosynthesis | 3.3 | 0.03 | 18.1 | B4GALT3, FUT8 |
| DistEndom | ||||||
| 1 | KEGG_91 | Rap1 signaling pathway | 5.6 | 0.003 | 7.3 | ARAP3, KIT, PLCB4, FGF17, FGF9, FGFR1, FLT4, ITGAL, SKAP1, CALM1 |
| ProxEndom | ||||||
| 1 | KEGG_103 | Steroid biosynthesis | 5.7 | 0.0002 | 20.7 | TM7SF2, LIPA, FAXDC2, CYP51 |
| 2 | KEGG_91 | Rap1 signaling pathway | 3.1 | 0.04 | 9.5 |
FGFR1, KIT, ANGPT2, FGFR2, SIPA1L2, GNAS, ITGAL, PLCB1, IGF1 EFNA4, RAPGEF2, LPAR3, LPAR2 |
| 3 | KEGG_250 | Glycosaminoglycan degradation | 3 | 0.05 | 20 | GUSB, HEXA, NAGLU |
| UTJ | ||||||
| 1 | KEGG_91 | Rap1 signaling pathway | 6.5 | 0.001 | 12.4 |
FIGF, ARAP2, IGF1R LPAR1, KIT, RRAS, INSR, AKT3, ITGAL, MAP2K1, PLCB4, TLN1, IGF1, FGF1, SRC, SKAP1, CTNND1 |
| 2 | KEGG_49 | Adherens junction | 5.7 | 0.003 | 17.4 | CSNK2A2, IGF1R, INSR, SORBS1, SRC, WASF1 |
| 3 | KEGG_139 | GnRH signaling pathway | 3.3 | 0.03 | 12.3 | FSHB, CAMK2G, MAP2K1, PLCB4, GNRHR2, SRC, MAP3K3 |
| 4 | KEGG_34 | Focal adhesion | 3.2 | 0.04 | 9.7 | FIGF, LAMA4, VAV2, IGF1R, THBS3, ITGA8, AKT3, PAK7, MAP2K1, TLN1, IGF1, SRC |
| Isth | ||||||
| 1 | KEGG_110 | N-glycans biosynthesis | 4.2 | 0.01 | 21.6 | ALG8, B4GALT1, DAD1, DPM1, MAN2A1, MGAT2, MOGS, STT3A |
| 2 | KEGG_252 | mTOR signaling pathway | 4.1 | 0.01 | 20 | AKT3, IGF1, MLST8, PIK3CD, PRKAA2, PRKCA, RRAGC, ULK1, VEGFA |
| Inf | ||||||
| 1 | KEGG_66 | FoxO signaling pathway | 5.8 | 0.002 | 25.8 | BCL6, BRAF, CCNB3, CCNG2, CDKN2D, EP300, FOXO3, GADD45G, IGF1, IGF1R, INSR, IRS1, MAPK10, MDM2, PCK2, PIK3R2, PLK3, PLK4, PRMT1, SIRT1, SOS2, STK11, TGFBR1, TNFSF10 |
| 2 | KEGG_59 | Cell cycle | 4 | 0.01 | 24.3 | BUB3, CCNB3, CCNH, CDC45, CDK1,CDKN2D, E2F1, E2F4, E2F5, EP300, ESPL1, GADD45G, MDM2, PTTG1, TP53, YWHAG, YWHAH, YWHAQ |
| 3 | KEGG_110 | N-glycans biosynthesis | 3.4 | 0.03 | 24.5 | ALG13, DAD1, DDOST, DOLPP1, DPM3, MAN1A1, MGAT4A, MGAT5, MOGS, STT3A |
| 4 | KEGG_195 | HIF-1 signaling pathway | 3 | 0.04 | 22.1 | ARNT, CAMK2D, EGLN1, EIF4E, EP300, EPO, GAPDH, IGF1, IGF1R, INSR, LTBR, NFKB1, PDHB, PDHB, PIK3R2, PLCG1, TEK, VHL |
*Upregulated genes are marked in bold; the unmarked were downregulated.
Endocervix (EndoCvx), Endometrium (DistEndom, ProxEndom), and endosalpinx from Utero-tubal Junction (UTJ), Isthmus (Isth), Infundibulum (Inf).