Table 6.
List of the most significant differently expressed biological pathways (P < 0.05) examined with KEGG database in artificial deposition of sperm-free seminal plasma (SP) from P1-fraction (SP-P1) group, in comparison with the Control group.
| PATHWAY ID | PATHWAY NAME | ENRICHMENT SCORE | P-VALUE | GENES ALTERED% | GENE LIST | |
|---|---|---|---|---|---|---|
| EndoCvx | ||||||
| 1 | KEGG_105 | Cell adhesion molecules | 3.7 | 0.02 | 5.5 | CD8B, SLA-1, SLA-DOA, SLA-DQB1, VCAM1 |
| 2 | KEGG_110 | N-glycans biosynthesis | 3.5 | 0.03 | 8.1 | ALG13, ALG5, B4GALT3 |
| DistEndom | ||||||
| 1 | KEGG_133 | Mismatch repair | 4.9 | 0.007 | 31.2 | AKT2, ATM, BCL2L1, CSF2RB, IRAK1, MYD88, PPP3CB, RELA, RIPK1, TNFRSF1A |
| 2 | KEGG_56 | Apoptosis | 4.7 | 0.082 | 19.2 | LIG1, MSH2, MSH3, PMS2, SSBP1 |
| ProxEndom | ||||||
| 1 | KEGG_213 | PI3K-Akt signaling pathway | 5.6 | 0.003 | 8 | ANGPT1, ANGPT2, CDK6, COL1A2, COL3A1, COL5A2, COL5A3, COL6A3, EPO, FLT1, IFN-ALPHA-9, ITGA5,, LAMA4, LPAR3, PGF, PIK3R5, PPP2CB, PRLR |
| 2 | KEGG_34 | Focal adhesion | 3.6 | 0.02 | 8.1 | COL1A2, COL3A1, COL5A2, COL5A3, COL6A3, FLT1, ITGA5, LAMA4, PGF, PIK3R5 |
| 3 | KEGG_195 | HIF-1 signaling pathway | 3.4 | 0.03 | 9.1 | EPO, NPPA, PIK3R5, TFRC |
| UTJ | ||||||
| 1 | KEGG_139 | GnRH signaling pathway | 5.1 | 0.006 | 8.8 | ATF4, GNRHR2, GRB2, MAP2K1, SOS2 |
| 2 | KEGG_46 | Estrogen signaling pathway | 4.8 | 0.008 | 8.1 | ATF4, GRB2, MAP2K1, SHC1, SOS2 |
| Isth | ||||||
| 1 | KEGG_34 | Focal adhesion | 5.6 | 0.003 | 12.2 | AKT3, BRAF, FYN, IBSP, ITGA2B, ITGA4, JUN, LAMA4, MRLC2, MYLK, MYLK2, PTEN, SHC4, SOS2, VCL |
| 2 | KEGG_98 | Rap1 signaling pathway | 5.5 | 0.004 | 11.7 |
AKT3, ARAP3, BRAF, CNR1, FGF17, FGF9, GNAO1, GNAQ INSR, ITGA2B, KRIT1, LCP2, P2RY1, RAPGEF6, RASGRP3, RGS14 |
| 3 | KEGG_66 | FoxO signaling pathway | 5.1 | 0.005 | 12.9 | AKT3, ATM, BRAF, CDKN1A, CDKN2B, FOXO3, INSR, PRKAA2, PTEN, SIRT1, SMAD4, SOS2 |
| 4 | KEGG_213 | PI3K-Akt signaling pathway | 4.9 | 0.007 | 9.8 | AKT3, CDKN1A, CRTC2, FGF17, FGF9, FOXO3, GHR, GNG13, IBSP, IL2RG, INSR, ITGA2B, ITGA4, JAK2, LAMA4, OSM, PRKAA2, PTEN, SOS2, SYK, TP53, YWHAQ |
| 5 | KEGG_46 | Estrogen signaling pathway | 3.9 | 0.02 | 13.1 | AKT3, FKBP4, GNAO1, GNAQ, JUN, MMP9, SHC4, SOS2 |
| 6 | KEGG_59 | Cell cycle | 3.8 | 0.02 | 12.2 | ATM, CCNH, CDKN1A, CDKN2B, RB1, SMAD4, STAG2, TP53, YWHAQ |
| 7 | KEGG_252 | mTOR signaling pathway | 3.2 | 0.03 | 13.3 | AKT3, BRAF, PRKAA2, PTEN, RICTOR, RRAGC |
| 8 | KEGG_49 | Adherens junction | 3.1 | 0.04 | 13 | FYN, INSR, SMAD4, VCL, WASF1, WASL |
| Inf | ||||||
| 1 | KEGG_195 | HIF-1 signaling pathway | 4.2 | 0.01 | 15.6 | ANGPT4, EGLN2, ENO3, GAPDH, IFNGR1, IL-6, LTBR, MTOR, PIK3R2, PLCG1, RELA, TEK |
*Upregulated genes are marked in bold; the unmarked were downregulated.
Endocervix (EndoCvx), Endometrium (DistEndom, ProxEndom), and endosalpinx from Utero-tubal Junction (UTJ), Isthmus (Isth) and Infundibulum (Inf).