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. 2020 Mar 19;10:5012. doi: 10.1038/s41598-020-61984-2

Table 1.

Summary of Western Blot results from red-eared slider and loggerhead gonad samples.

Incubation Treatment Sox9 Dmrt1 AMH CIRBP Aromatase Males Females
T. scripta MPT (N = 5) 5/5 5/5 5/5 5/5 0/5 5 0
FPT (N = 5) 0/5 0/5 0/5 5/5 5/5 0 5
C.caretta MPT (N = 2) 2/2 2/2 2/2 2/2 0/2 2 0
FPT (N = 3) 0/3 0/3 0/2 3/3 3/3 0 3

Sex was verified independently via H&E histological sections. All gonads were collected from 1–2 day old hatchlings. Note that CIRBP was detected in gonad samples from both male and female hatchlings. The numerators indicate how many of the individuals tested were positive for the protein.