Table 1.
Incubation Treatment | Sox9 | Dmrt1 | AMH | CIRBP | Aromatase | Males | Females | |
---|---|---|---|---|---|---|---|---|
T. scripta | MPT (N = 5) | 5/5 | 5/5 | 5/5 | 5/5 | 0/5 | 5 | 0 |
FPT (N = 5) | 0/5 | 0/5 | 0/5 | 5/5 | 5/5 | 0 | 5 | |
C.caretta | MPT (N = 2) | 2/2 | 2/2 | 2/2 | 2/2 | 0/2 | 2 | 0 |
FPT (N = 3) | 0/3 | 0/3 | 0/2 | 3/3 | 3/3 | 0 | 3 |
Sex was verified independently via H&E histological sections. All gonads were collected from 1–2 day old hatchlings. Note that CIRBP was detected in gonad samples from both male and female hatchlings. The numerators indicate how many of the individuals tested were positive for the protein.