Figure 4.
B cell-dependency in PLPECD-driven EAE disease in B6 mice occurs irrespective of antibody production. (A) WT, μMT, and AID−/−μS−/− mice were immunized s.c. with 100ug PLPECD/CFA on day 0 and given 250 ng pertussis toxin i.p. on days 0 and 2. Clinical disease scores were monitored and EAE parameters were analyzed (depicted in table as mean values ± standard error of the mean). (B) On day 23 post-immunization, groups of mice in A were challenged with PLP178–191 or PBS alone in right and left ear pinnae, respectively. Ears were measured at 48 h. ns = not significant; *p < 0.05; ***p < 0.001; ****p < 0.0001.