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. 2020 Mar 19;10:5076. doi: 10.1038/s41598-020-61797-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) The nuclear area of folate deprived cells was assessed by fluorescence microscopy after phalloidin-tetramethylrhodamine B isothiocyanate and Hoechst 33342 staining. Merged images of tubulin and DNA are shown. Scale bars represent 100 µm. (B) Using ImageJ software (National Institute of Mental Health, Bethesda, Maryland, USA) the nuclear area of 100 fibroblasts in 10 different fields was calculated. Values represent the mean ± SEM. Different letters indicate significant differences (P < 0.05).