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. 2020 Mar 19;11:1473. doi: 10.1038/s41467-020-15324-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a The main biosynthetic pathway of caffeine (4) from xanthosine (1). b Conversion of caffeine to 1,3,7-trimethyluric acid does not progress to theacrine in Puer leaves. c The content of caffeine (4), 1,3,7-trimethyluric acid (5) and theacrine (6) in Puer and Kucha leaves. The left panel shows the HPLC analysis of 4 and 6 at absorbance wavelength of 254 nm. The right three panels show the quantification of 4, 5, and 6 by HPLC-MS. Data represents mean ± SD (n = 4). The corresponding dot plots are overlaid on the figure. d Conversion of caffeine to 1,3,7-trimethyluric acid then to theacrine in Kucha leaves.