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. 2020 Mar 19;11:1473. doi: 10.1038/s41467-020-15324-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Crystal structure of CkTcS dimer. SAH and 1,3,7-trimethyluric acid (5) are shown in ball-and-stick representation. b Structural overlap of CkTcS (pink) with DXMT (blue) and XMT (grey), with the major structural difference around the substrate binding pocket between CkTcS, DXMT, and XMT highlighted. c A close-up view of the CkTcS-1,3,7-trimethyluric acid interactions. The hydrogen bonds are shown in red dashed lines. d A close-up view of the π–π-stacking interactions. e In vitro methylation assay of wild-type (WT) or mutants of CkTbS using 1,3,7-trimethyluric acid as substrate, with CkTcS as positive control. Data represent mean ± SD (n = 3). The corresponding dot plots are overlaid on the figure. The experiment was repeated twice. Differences were assessed statistically by two-tailed Student’s t-test, ^****P < 0.0001, ^*P < 0.05.