Figure 2.

Biochemical properties of Ni-binding capabilities. (a) Skin-draining LN cells were incubated with 100 μM of NiCl₂ for 60 min followed by 1 mM EDTA for 30 min. Histograms represent the NPG fluorescence of migratory DCs. Numbers shown in parentheses are the MFI of each histogram. Results are representative of three independent experiments. Graph showing ΔMFI. Bars represent the mean ± SD of three independent experiments. Each symbol represents the value from an independent experiment. (b) Skin-draining LN cells were incubated with 100 μM NiCl₂ for 60 min with or without divalent cations at 500 μM. Results are shown as ΔMFI. Bars represent the mean ± SD of three independent experiments. Each symbol represents the value from an independent experiment. (c) Skin-draining LN cells were treated with trypsin for the indicated time, then incubated with 100 μM NiCl₂ for 60 min followed by NPG. Results are shown as the ΔMFI of NPG, MFI of MHC class II and CD11c. Bars represent the mean ± SD of three independent experiments. Each symbol represents the value from an independent experiment. *P < 0.05, **P < 0.01, significantly different from the control (0 min). (d) Skin-draining LN cells were incubated with anti-MHC class II Abs for 20 min on ice, then incubated with 100 μM NiCl₂ for 60 min followed by NPG. Results are shown as the ΔMFI of NPG. Bars represent the mean ± SD of three independent experiments. Each symbol represents the value from an independent experiment.