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. 2020 Mar 19;10:5050. doi: 10.1038/s41598-020-61875-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Ni-NPG staining of DCs from various LNs. (a) Cells were incubated with 100 μM NiCl₂ for 60 min followed by NPG. Histograms represent the NPG fluorescence of cells incubated with (black line) or without (gray-shaded) NiCl₂. Results are representative of three independent experiments. (b and c) Ni-NPG staining of migratory (b) and resident (c) DCs was analyzed. Results are shown as ΔMFI. Bars represent the mean ± SD of three independent experiments. Each symbol represents the value from an independent experiment. Means without a common letter were significantly different, P < 0.05.