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. Author manuscript; available in PMC: 2020 Mar 19.
Published in final edited form as: Cancer Res. 2017 Sep 21;77(22):6168–6178. doi: 10.1158/0008-5472.CAN-17-0530

Figure 2. miR-130a promotes cell proliferation.

Figure 2.

(A) Expression levels of miR-130a in E1A- or E1A/iRas-transduced MEFs cultured with ± Dox for 2 days were determined using TaqMan miRNA assays. (B-E) E1A/H-RasV12 transformed MEFs were infected with miR-130a expression vector or control vector. miR-130a expression levels were detected by TaqMan miRNA assay after the MEFs were treated with ± Dox for 2 days (B). Cell number (C), BrdU incorporation (green) indicated by red arrows after 5 hrs uptake of BrdU (D), and apoptosis by Annexin V assay (E), were determined. DAPI was used for counterstaining. Bar, 50 μm.