. 2020 Feb 18;32(2):184–191. doi: 10.1177/1040638720906814

Table 2.

Primers and PCR methods of Clostridium tetani detection in biological or environmental samples. Enrichment culture and subsequent DNA extraction are commonly used prior to PCR amplification.

Primer	Sequence	PCR	Reference
P476	ATGCCAATAACCATAAATAATTTTAGATATAG	Conventional PCR	⁴³
P477	TTCATCTTGAAATGGTTCTTCTG	Conventional PCR	⁴³
F3	GATAAAGATGCATCTTTAGGATT	LAMP PCR	^30,41
B3	TCTTCTTCATTATCAACCCAAC
FIP	AGTTGCTTGCAATTAATATATCCCTAGTAGGTACCCATAATGGTCA
BIP	AACATGTGATTGGTACTTTGTACCTTATGTGTCTATGGTGTGTTG
TET1	CCTAGTTTCAAAACTTATTGGCTTATGTAA	Conventional PCR	²⁹
TET2	CATAATTCTCCTCCTAAATCTGTTAATGAT	Conventional PCR	²⁹
Forward	CTGGATTGTTGGGTTGATAATG	Conventional PCR	^25,42
Reverse	ATTTGTCCATCCTTCATCTGTAGG	Conventional PCR	^25,42
TQ TET1	CCTAGTTTCAAAACTTATTGGCTTATGTAA	Real-time PCR	²
TQ TET2	CATAATTCTCCTCCTAAATCTGTTAATGATG	Real-time PCR	²

LAMP = loop-mediated isothermal amplification.