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. 2020 Mar 19;13:38. doi: 10.1186/s13041-020-00573-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Differentiation of iPSC derived MCs. iPSCs were treated by GSK3β inhibitor, then StemPro34 and VEGF165, to obtain Flk-1 positive/VE-Cadherin negative mural cells (MCs, a). Differentiated mural cells were immature at the time of the sorting (b) and further cultured for maturation. MCs expressed VSMC marker αSMA, SM22 and calponin (c-e). Endothelial cells (ECs) can be also isolated as Flk-1 positive/VE-Cadherin positive cells. MCs differentiated by modified protocol changed their morphology depending on the culture condition (g and i, phenotypic transition), but not MCs differentiated by original protocol (f and h). Control MC (TIG107) differentiated in αMEM supplemented with 5%FBS and PDGF-BB showed markedly low PDGFRβ, irrespective of the coating (j, lane F and H). Bars represent 20 μm in (c-e), 150 μm in (b, f-i)