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In vivo validation: HULC knockdown inhibited osteosarcoma tumour growth and metastasis. Images of tumours from the nude mouse model (A); statistical analysis of tumour volume (B) and weight (C); images of lung tissues from the nude mouse model (D); qRT-PCR or western blot was performed to evaluate the relative mRNA or protein expression levels of HULC (E), miR-372-3p (F) and HMGB1 (G). Data are shown as the mean ± SD. * p < 0.05, ** p < 0.01
