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. 2020 Mar 18;17:22. doi: 10.1186/s12986-020-00443-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Effect of drinking water containing trehalose on intracytoplasmic lipid droplets in jejunal epithelium. Representative images (400x) of hematoxylin-eosin staining in sections of jejunum are shown (a) Oil Red O staining showing the presence of neutral lipids in jejunal intracytoplasmic vacuoles (b) intraepithelial lipid ratio (%) in the jejunal epithelium determined using the image software cellSens (c). Values are shown as means ± standard deviation (n = 7–8). Statistical analysis was performed using a Steel-Dwass test. The values indicate that the statistical significances were p < 0.05. KO/HFD groups vs. KO/CE-2/Water: #p < 0.05