Table 1.

Sequences of primers for msp1, msp2 and glurp genes of P. falciparum from Cameroon

Msp-1 (block 2)
Primary PCR	CHM1-OF: 5′ CTAGAAGCTTTAGAAGATGCAGTATTG-3′ CHM1-OR: 5′ CTTAAATAGTATTCTAATTCAAGTGGATCA-3′
Secondary PCR	CHM1-KF: 5′ AAATGAAGAAGAAATTACTACAAAAGGTGC-3′ CHM1-KR: 5′ GCTTGCATCAGCTGGAGGGCTTGCACCAGA-3′ CHM1-MF: 5′ AAATGAAGGAACAAGTGGAACAGCTGTTAC-3′ CHM1-MR: 5′ ATCTGAAGGATTTGTACGTCTTGAATTACC-3′ CHM1-RF: 5′TAAAGGATGGAGCAAATACTCAAGTTGTTG-3′ CHM1-RR: 5′ CATTTGAAGGATTTGCAGCACCTGGAGATC-3′
Msp-2 (block 3)
Primary PCR	M2-OF: 5′ ATGAAGGTAATTAAAACATTGTCTATTATA-3′ M2-OR: 5′ CTTTGTTACCATCGGTACATTCTT’3′
Secondary PCR	M2-FCF: 5′ ATATTAAGAGTGTAGGTGCARATGCTCCA-3′ M2-FCR: 5′ TTTTATTTGGTGCATTGCCAGAACTTGAAC-3′ M2-ICF: 5′AGAAGTATGGCAGAAAGTAAKCCTYCTACT-3′ M2-ICR: 5′ GATTGTAATTCGGGGGATTCAGTTTGTTCG-3′
Glurp (region II)
Primary PCR	CHG-OF: 5′ -TGAATTTGAAGATGTTCACACTGAAC-3′ 3′ CHG-OR: 5′ -GTG GAATTGCTTTTTCTTCAACACTAA-3′
Secondary PCR	CHG-NF: 5′-TGAATTTGA AGA TGT TCA CAC TGA AC-3′ CHG-OR: 5′-GTG GAATTGCTT TTTCTTCAACAC TAA-3′