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. 2020 Mar 12;8:164. doi: 10.3389/fbioe.2020.00164

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Crown Copyright © 2020 APHA. Konold, Spiropoulos, Thorne, Phelan, Fothergill, Rajanayagam, Floyd, Vidana, Charnley, Coates and Simmons

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot profile of digested brain samples from selected goats using two monoclonal antibodies. (A) Sha31 antibody. (B) P4 antibody. Lanes 1 and 10: molecular mass marker; lane 2: goat 2135 (clinical suspect, positive on brain by IHC and ELISA); lane 3: goat 2113, clinical suspect, positive on brain by IHC and ELISA); lane 4: goat 2078 (no clinical signs of scrapie, positive on brain by IHC, negative by ELISA); lane 5: goat 2117 (no clinical signs of scrapie, negative on brain by IHC and ELISA, positive on lymphoid tissue by IHC; scrapie profile difficult to discern with the picture contrast used); lane 6: goat 2102 (clinical suspect, negative on brain by ELISA and on all tissues by IHC); lane 7: caprine classical scrapie control (RSCRAP 17/00006, II₁₄₂QQ₂₂₂); lane 8: bovine classical BSE control (RBSE 98/00291); lane 9: ovine classical scrapie control (PG1903/97).