FIG 2.

Substitution of the residues involved in processing of the 1/2 site alters the G3BP sensitivity of SFV trans-replicase and virus. (A) Schematic representation of substitutions examined in the context of (B) trans-replicase and (C) virus. (B) U2OS WT or ΔΔ cells were cotransfected with indicated SFV replicases and corresponding templates. Fluc (replication) and Gluc (transcription) activities were measured 20 h posttransfection, normalized to signals obtained from cells transfected with catalytic-inactive replicases, and plotted as the fold change. Data are means of the results from three independent experiments. Error bars indicate the standard deviation (SD). Statistical differences were as follows: *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001. (C) U2OS WT or ΔΔ cells were infected with indicated SFV viruses at an MOI of 0.1, and viral titers at 24 hpi and 48 hpi were quantified by plaque assay. Data are means of the results from three independent experiments. Error bars indicate the SEM. Statistical differences were as follows: ns, not significant; *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001.