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CHIKV trans-replicase fails to produce dsRNA in the absence of G3BP. U2OS WT or ΔΔ cells were cotransfected with WT CHIKV or CHIKV P1*2*34 trans-replicase plasmids. At 24 h posttransfection, cells were fixed and stained for nsP3 (red) and double-stranded RNA (dsRNA) (green). Nuclei are shown in blue. Representative images from three independent experiments are shown.
