FIG 9.

G3BP deletion or the P4 Arg-to-His substitution does not prolong or increase ns protein translation. (A) Schematic representation of modifications present in the ns polyprotein region of the infectious clones CHIKV-WA-P3NanoLuc (WA) or CHIKV-WA-RH-P3NanoLuc (WA-RH). The NanoLuc luciferase sequence was inserted between the codons of amino acid residues 1716 and 1717. (B) U2OS WT cells, cultured at 28°C or 39°C, were infected with CHIKV-WA or CHIKV-WA-RH at an MOI of 1, and reporter activity was measured from lysates prepared at indicated time points. (C) U2OS WT or ΔΔ cells, cultured at 39°C, were transfected with in vitro-transcribed infectious RNA of CHIKV-WA or CHIKV-WA-RH. Reporter activity was measured from lysates prepared at the indicated time points. Data are the means of the results from three independent experiments. Error bars indicate the SD.