FIG 10.

Toxicity and inhibition of BIRFLU in human A549 cells. Confluent monolayers (96-well plates; 5.0 × 10⁴ cells/well; quadruplicates) of human A549 cells were infected with 200 FFU of BIRFLU. After 1 h of viral adsorption, the indicated concentrations (3-fold serial dilutions, starting concentration of 50 μM) of the indicated compounds or 0.1% DMSO vehicle control were added to the postinfection media. At 48 hpi, tissue culture supernatants from infected A549 cells were collected and used to measure Nluc expression. Images of Venus expression were taken using a fluorescence microscope. Percent viral infection and the EC₅₀ were calculated based on Nluc expression. Dotted lines indicate 50% viral inhibition. Data are expressed as mean and SD from three independent experiments conducted in quadruplicates. Bar, 50 μm.