FIG 8.

DPM1 deficiency affects E and prM glycoprotein epitope accessibility. Control, DPM1^KO, and STT3A^KO 293T cells were cotransfected with DENV C-prM-E and NS2B-NS3 plasmids (1:1 ratio). (A) Relatively equivalent amounts of viral glycoproteins were immunoprecipitated with MAb 4G2, followed by immunoblot analysis with anti-E antibodies. (B) Relatively equivalent amounts of viral glycoproteins were immunoprecipitated with MAb 2H2, followed by immunoblot analysis with anti-prM antibodies. (C) Control, DPM1^KO, and DPM3^KO 293T cells were transfected with DENV HA-NS1 plasmid. Relatively equivalent amounts of viral glycoproteins were immunoprecipitated with commercially available (Abcam; ab41623) anti-NS1, followed by immunoblot analysis with anti-HA antibody. Blots are representative of those from three independent experiments. Bar graphs represent quantification of the chemiluminescent band intensities relative to E, prM, and NS1 expression in control cells. Each point plotted corresponds to the quantification from one transfection experiment. Data are means ± SD from from three (A and B) or two (C) independent transfection experiments. Significance was calculated using a one-way ANOVA with Dunnett’s multiple-comparison test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.