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Diagram indicating the position of the I177L open reading frame in the ASFV-G genome (top). A donor plasmid with arms homologous to ASFV-G and the mCherry under the control of the p72 promoter was used to introduce the final genomic changes (bottom) to create ASFV-Georgia-ΔI177L, where the sequence of the donor plasmid mCherry reporter (red) is introduced to replace the ORF of I177L, as indicated.
