EV-D68 infection induces the formation of SGs during the early stage and inhibits the formation of SGs during the late stage. (A, B, C, D, E, and F) RD/HeLa cells were infected with EV-D68 (MOI of 1), fixed at 0, 4, 8, 12, and 24 h, and then stained with a rabbit anti-TIA1 monoclonal antibody (A and D), a rabbit anti-G3BP1 monoclonal antibody (B and E), or a rabbit anti-HUR monoclonal antibody (C and F) for immunofluorescence. (G, H, I, J, K, and L) Quantitative analysis of the number of SGs in each cell.