FIG 1.

The cross-antigenicity characterization of the five FiVs, TUHV, and RABV. (A) Phylogeny and pairwise comparison of the 340-aa NP C-terminal sequences. Viruses in phylogenetic tree showing significant cross-reactivity are in grayed boxes with the depth of gray representing the reactive strength. (B) WB analysis of eukaryotically expressed NPs of the 7 viruses using 6 antiserum samples. Lanes were taken from different gels. Different loading amounts of these NPs were determined using ImageJ to ensure the same amounts of the target proteins. Z, EBOV (loading amount, 52.3 μg); R, RESTV (59.5 μg); D, FiV DH04 (95.55 μg); M, MARV (60.8 μg); L, MLAV (51.8 μg); T, TUHV (48.6 μg); C, RABV CVS11 (60.9 μg); E, EGFP (11.0 μg).