KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| anti-MBNL1 | EMD Millipore Corporation | CAT# MABE70 |
| anti-mouse IgG Dylight 488 | Thermo Scientific Pierce | CAT# PI35502 |
| anti-mouse IgG horseradish-peroxidase | Cell Signaling Technology | CAT# 7076 |
| anti-rabbit IgG Vinculin (E1E9V) | Cell Signaling Technology | CAT# 13901 |
| anti-rabbit IgG horseradish-peroxidase | Cell Signaling Technology | CAT# 7074 |
| Critical Commercial Assays | ||
| Quick-RNA Miniprep Kit | Zymo | CAT# R1055 |
| qScript cDNA synthesis | Quanta BioSciences | CAT# 101414–100 |
| SYBR Green Master Mix | Life Technologies | CAT# 4368708 |
| GoTaq DNA polymerase | Promega | CAT# M8295 |
| Oligonucleotides | ||
| Control siRNA-A | Santa Cruz Biotechnology | CAT# sc-37007 |
| siRNA-MBNL1 | Dharmacon | N/A |
| Primers used for RT-qPCR; see Table S1 | Integrated DNA Technologies/ Eurofins MWG Operon | N/A |
| 5’-Cy3-(CAGG)10 | Margolis et al., 2006; Integrated DNA Technologies | N/A |
| Software and Algorithms | ||
| ImageJ | NIH | N/A |
| Olympus Fluoview software version 3.0 | Olympus | N/A |
| GraphPad Prism 7 | GraphPad Prism Software, Inc. | N/A |