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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Acc Chem Res. 2019 Sep 5;52(10):2841–2848. doi: 10.1021/acs.accounts.9b00386

Figure 5.

Figure 5.

Next-generation ABS fluorescence probes for FA that share a general activity-based trigger operating through tandem aza-Cope rearrangement and β-elimination reactions. (a) (top) Reaction scheme for the detection of FA by the generalizable FA trigger and (bottom) structures of a color palette of FA probes. (b) Confocal microscopy images of wild-type (WT) HEK293T cells stained with FAP573 (10 μM) for 60 min in the presence (1000 μM) or absence of FA. (c) Confocal microscopy images of near-haploid (HAP-1) alcohol dehydrogenase knockout (Adh5 −/−) cells stained with FAP573 (10 μM) for 60 min in the presence (100 μM) or absence of FA. In (b) and (c), bar graphs present fluorescence intensities (mean ± SEM, n = 3; ***, P < 5 × 10−5). Adapted from ref 45. Copyright 2017 American Chemical Society.