Figure 1. Rapid Induction of KLRG1⁺ and CD57⁺ surface expression in CMV specific CD8 T_AEFF during acute primary CMV infection evolve into T_EM during chronic infection.

(A) Representative flow cytometric plots show the expression of KLRG1⁺ (upper panel) and CD57⁺ (lower panel) in total CD8⁺ T cells from D+R-patient during Pre-CMV and Acute CMV infection. Total CD8⁺ T cells were gated on live CD3⁺ T cells after excluding CD14⁺, CD16⁺, and CD19⁺ cells. (B) Cumulative data showing distributions of total KLRG1⁺CD8⁺ (left graph) and CD57⁺CD8⁺ (right graph) T cells from patients during Pre-CMV and Acute CMV infection (n=8). Statistical analysis was performed using Wilcoxon matched pair signed rank test. Also Mann-Whitney U test was performed with a two-sided and p value of less than 0.05 considered statistically significant. (C) Cumulative data shows distributions of total CD3⁺CD8⁺ T cells KLRG1⁺ and CD57⁺ for (n=23) patients during Acute CMV) infection. (D) Representative flow cytometric plots show expression of CD57⁺ and CD27⁺ on CD8⁺ T cells during Pre-CMV (left plot) and Acute CMV (right plot). (E) Representative flow cytometric plots showing co-expression of CD57⁺ and CD27⁺ (upper panels) or KLRG1⁺ and CD57⁺ (lower panels) on the ^gated population of total CD3⁺CD8⁺ T cells ex vivo from D+R-patient during Acute CMV (left plots) and Chronic CMV (right plots) infection. (F) Cumulative data graph showing frequency of total CD8⁺ T cells expressing phenotypic markers as labeled. PBMCs from acute CMV (orange circles) and chronic CMV (green squares) (n=14 dextramer responses) D+R-patients were stained ex vivo and analyzed by flow cytometry. (G) Representative plots show CD57⁺ and KLRG1⁺ expression on MHC class I dextramer cells and co-expression of CD57⁺, and CD27⁺ or KLRG1⁺ on the gated population dextramer⁺ cells CD3⁺CD8⁺ T cells. (H) Cumulative data graph shows proportions of total CD8⁺ T cells proportions of MHC class I dextramer ⁺ cells from acute CMV (n=14 dextramer responses) (orange circles) and chronic CMV (n=14 dextramer responses) (green squares) infection, expressing distinct phenotypic markers are shown.