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. 2020 Mar 19;18(3):e3000651. doi: 10.1371/journal.pbio.3000651

Fig 4. Validation of nuc-aAST using clinical isolates.

Fig 4

(a-c) nuc-aAST results after 15 min of exposure to (a) PEN, (b) CFM, and (c) CRO. (d-e) nuc-aAST results after exposure to (d) CFM and (e) CRO for 30 min. Each point represents the average for a single isolate run in (at least) biological triplicate for that condition. All PCR was performed in technical triplicate. The dashed line represents the susceptibility threshold, which was set at 26.5% accessibility for 15-min exposures and 46% for 30-min exposures. Data plotted are in S4 Table; experimental data from individual replicates are detailed in S11 Table and S12 Table. AST, antibiotic susceptibility testing; CFM, cefixime; CRO, ceftriaxone; nuc-aAST, nuclease-accessibility AST; PEN, penicillin; R, resistant; S, susceptible.