Fig 1. Binding studies to assess dual specificity for the junctional and NANP epitopes.

(A). Biolayer Interferometry (BLI) was used to measure the kinetics of binding for Fab317, Fab667 and Fab668 to either the NPNANPNANPNANPNA (NANP) peptide or the KQPADGNPDPNANPNV (junctional) peptide. Binding curves are shown in light purple (wider lines) and fits are shown in dark purple (thinner lines). As the fits to the data are very good, the binding curves and fits overlap. (B) Alanine scan for the junctional peptide using BLI. The binding response (amplitude) is normalized to the wild-type junctional peptide. Binding is shown as a gradient from dark purple (native binding) to white (no binding).