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. 2020 Mar 9;16(3):e1008646. doi: 10.1371/journal.pgen.1008646

Table 1. Centromeres identified in the Psojae2019.1 assembly and their counterparts in the Sanger assembly.

Psojae2019.1 Sanger V3
Name Contig Position of core centromere, kb
(size, kb)*
GC% of CEN Position of pericentric region, kb (size, kb) Scaffold Position of core centromere (kb)*
CEN1 1 415–578 (163) 56.91 361–415 (54)
579–650 (71)
2 7086–7267
CEN2 2 4102–4295 (193) 55.51 4094–4102 (8)
4296–4305 (9)
8 2374–2420
CEN3 3 3138–3410 (272) 54.81 3223–3138 (15)
3412–3560 (48)
9 3075–3286
CEN4 11 991–1174 (183) 58.13 944–991 (47)
1175–1205 (30)
4 995–1246
CEN5 18 1556–1706 (150) 57.93 1492–1556 (64)
1709-? (>22)
3 4078–4138
CEN6 34 697–880 (183) 57.65 643–696 (53)
882–904 (22)
6 1521–1726
CEN7 36 432–706 (274) 52.16 376–433 (57)
708–732 (24)
5 2049–2310
CEN8 38 302–490 (188) 57.01 288–302 (14)
490–515 (25)
1 9667–9688
CEN9 41 154–342 (188) 57.40 101–153 (52)
342–358 (16)
1 2921–3079
CEN10 51 36–151 (115) 57.93 ?-36 (>36)
152–216 (64)
- -

* The coordinates of core centromeres in the Psojae2019.1 assembly are defined by peak calling (S7 File). The positions of centromeres in the Sanger assembly are defined by visualization of peaks.

Contig11 contains a minor (coordinate, 991,465–1,009,035, 18 kb) and a major (coordinate, 1,060,806–1,174,302, 114 kb) peaks that are separated by a 21 kb transcriptionally active region. The core centromere size is defined by the combination of the minor and major CENP-A regions, and the region between the two peaks.

One side of pericentric heterochromatin region is not fully assembled.