Table 1.

Dissociation Constants (K_D) for Homodimerization of δ-protocadherin Extracellular Regions and Fragments Determined by Analytical Ultracentrifugation

Protein	Schematic	Oligomeric State	Monomer Mw (kDa)a	Apparent Mw (kDa)b	Kd (µM)
δ1-family
Pcdh-1		dimer	95.7	177 ± 3.4	1.2 ± 0.55c
Pcdh-7moused		dimer	101.6	168 ± 4.9	8.2 ± 2.5
Pcdh-9		dimer	103.5	204 ± 3.7	2.03 ± 0.43
Pcdh-11e		dimer	113.5	190 ± 1.4	7.8 ± 0.17
δ2-family
Pcdh-8		monomer	78.7	83.1 ± 0.7	N/A
Pcdh-8mouse		monomer	79.4	78.3 ± 0.2	N/A
Pcdh-8.1Xenopus		dimer	76.7	134.7 ± 5.9	3.5 ± 2.4
Pcdh-8.2Xenapus		dimer	76.6	138.2 ± 0.9	1.7 ± 0.10
Pcdh-10		dimer	77.2	134 ± 2.0	3.6 ± 0.61
Pcdh-12		dimer	78.8	133 ± 0.1	5.4 ± 0.20
Pcdh-17		dimer	87.3	168 ± 4.9	4.8 ± 0.54
Pcdh-18		dimer	82.0	148 ± 2.8	2.7 ± 0.67
Pcdh-19		dimer	78.0	139 ± 3.8	0.48 ± 0.10
Fragments
Pcdh-1 EC1-EC4		dimer	50.5	97.7 ± 2.2	0.53 ± 0.46
Pcdh-7 EC1-EC4		dimer	56.5	104 ± 1.5	2.70 ± 1.0
Pcdh-7 EC1-EC4mouse		dimer	56.4	101 ± 1.4	4.1 ± 1.3
Pcdh-9 EC1-EC4		dimer	56.1	104 ± 1.5	2.0 ± 0.52
Pcdh-10 EC1-EC4		dimer	50.2	96.4 ± 0.4	0.68 ± 0.13
Pcdh-1 EC1-EC3		monomer	35.9	36.8 ± 0.9	N/A
Pcdh-10 EC1-EC3		weak dimer	39.5	45.8 ± 1.4	280 ±110
Pcdh-1 EC5-EC7		monomer	47.1	45.8 ± 0.04	N/A
Pcdh-10 EC5-EC6		monomer	28.7	29.4 ± 0.2	N/A
Pcdh-8 EC5-EC6		monomer	26.9	27.8 ± 0.1	N/A

See also Figure S2.

^aAverage molecular weight determined from mass spectrometry peak maximum.

^bDetermined from AUC data using an ideal monomer model.

^cErrors represent standard deviation from two or more experiments.

^dAll constructs derive from human except where stated.

^ePcdh11Y isoform is reported.