KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Non-clustered protocadherin proteins	This paper	N/A
Clustered protocadherin proteins	Rubinstein et al., 2015; Goodman et al., 2016a, 2016b; this paper	N/A
Polyethylenimine	Polysciences	Cat# 24765–2
Freestyle 293 Expression Media	Thermo Fisher Scientific	Cat# 12338–018
Opti-MEM Reduced Serum Media	Thermo Fisher Scientific	Cat# 31985–070
IMAC Sepharose 6 Fast Flow	GE Healthcare	Cat# 17092109
Biotin	Sigma	Cat# B4501
NeurAvidin-HRP	Thermo Fisher Scientific	Cat# 31030
Tris Base	Fisher Scientific	Cat# BP152–5
Sodium Chloride	Fisher Scientific	Cat# S271–10
Calcium Chloride Dihydrate	JT Baker	Cat# 1336–01
Imidazole	ACROS	Cat# 301870025
NeutrAvidin protein	Thermo Fisher Scientific	Cat# 31000
HEPES	Sigma	Cat# H3375
Tween-20	Sigma	Cat# P7949
BSA	Sigma	Cat# A7906
Series S CM4 chip	GE Healthcare	Cat# BR100539
Fetal Bovine Serum	Thermo Fisher Scientific	Cat# 16141079
DMEM with GlutaMAX	Thermo Fisher Scientific	Cat# 10569010
Penicillin Streptomycin	Thermo Fisher Scientific	Cat# 15070063
Glycerol	ACROS	Cat# 332031000
PEG 4,000	ACROS	Cat# 434630010
Bicine	Molecular Dimensions	Cat# MD2–003
Morpheus monosaccharides mix	Molecular Dimensions	Cat# MD2-100-75
PEG 8,000	Sigma	Cat# 89510
Ethylene Glycol	Fluka	Cat# 03760
Morpheus NPS mix	Molecular Dimensions	Cat# MD2-100-72
MES	Sigma	Cat# M3671
PEG 20,000	Sigma	Cat# 81300
PEG 550 monomethyl ether	Sigma	Cat# 202487
PEG 400	Sigma	Cat# 91893
Sodium Acetate	Sigma	Cat# S7545
Bicine	Sigma	Cat# B3876
Glutaraldehyde	Sigma	Cat# G5882
(2R,3R)-(–)-2,3-Butanediol	Sigma	Cat# 237639
DOPC lipids	Avanti Polar Lipids	Cat# 850375C
DOGS-NTA lipids	Avanti Polar Lipids	Cat# 790404C
Rhodamine-DHPE lipids	Thermo Fisher Scientific	Cat# L1392
Potassium chloride	Sigma	Cat# P3911
300 mesh copper EM grids	Electron Microscopy Services	Cat# EMS300-Cu
Cellulose acetate	Sigma	Cat# 180955
Ethyl acetate	EMD	Cat# EX0241–6
Critical Commercial Assays
Spin Miniprep Kit	QIAGEN	Cat# 27106
Hispeed Plasmid Maxi Kit	QIAGEN	Cat# 12663
SF Cell Line 4D-Nucleofector® X Kit S	Lonza	Cat# V4XC-2032
Deposited Data
Crystal structure of human pcdh1 EC1–4	This paper	PDB ID: 6VFP
Crystal structure of human pcdh10 EC1–4 monomer	This paper	PDB ID: 6VFQ
Crystal structure of human pcdh10 EC1–4 dimer	This paper	PDB ID: 6VFW
Crystal structure of human pcdh17 EC1–4	This paper	PDB ID: 6VFT
Crystal structure of human pcdh18 EC1–4	This paper	PDB ID: 6VFR
Crystal structure of human pcdh19 EC1–4	This paper	PDB ID: 6VFU
Crystal structure of human pcdh8 EC5–6	This paper	PDB ID: 6VFV
Crystal structure of Xenopus pcdh8.1 EC1–6	This paper	PDB ID: 6VG1
Crystal structure of human pcdh10 EC1–6	This paper	PDB ID: 6VG4
Human δ pcdh1 ectodomains on membranes, tomogram 1	This paper	EMDB: EMD-21188
Human δ pcdh1 ectodomains on membranes, tomogram 2	This paper	EMDB: EMD-21189
Human δ pcdh1 ectodomains on membranes, tomogram 3	This paper	EMDB: EMD-21190
Human δ pcdh10 ectodomains on membranes, tomogram 1	This paper	EMDB: EMD-21191
Human δ pcdh10 ectodomains on membranes, tomogram 2	This paper	EMDB: EMD-21192
Human δ pcdh10 ectodomains on membranes, tomogram 3	This paper	EMDB: EMD-21193
Experimental Models: Cell Lines
Human: FreeStyle 293-F cells	Thermo Fisher Scientific	Cat# R79007
Human: K-562 bone marrow chronic myelogenous leukemia (CML) cells	ATCC	Cat# ATCC CCL-243
Recombinant DNA
VRC-8400 vector	Vaccine Research Center (NIH), Gary Nabel	N/A
pmax-mCherry vector	Thu et al., 2014	N/A
BirA plasmid	Harrison et al., 2016	N/A
Human pcdh1 cDNA	DNASU	Cat# HsCD00399247
Mouse pcdh7 cDNA	Transomic	Cat# BC131967
Human pcdh9 cDNA	DNASU	Cat# HsCD00516491
Human pcdh11Y cDNA	DNASU	Cat# HsCD00083030
Human pcdh8 cDNA	DNASU	Cat# HsCD00045356
Human pcdh10 cDNA	DNASU	Cat# HsCD00505992
Human pcdh12 cDNA	DNASU	Cat# HsCD00641460
Human pcdh17 cDNA	Dr. Chan Aye Thu	N/A
Human pcdh18 cDNA	Dr. Chan Aye Thu	N/A
Human pcdh19 cDNA	Dr. Chan Aye Thu	N/A
Human pcdh20 cDNA	DNASU	Cat# HsCD00399990
Xenopus pcdh8.1 cDNA	Transomic	Cat# BC074360
Xenopus pcdh8.2 (PAPC) cDNA	DNASU	Cat# XLCD00716163
Software and Algorithms
XDS	Kabsch, 2010	http://xds.mpimf-heidelberg.mpg.de
CCP4	Winn et al., 2011	http://www.ccp4.ac.uk/
AIMLESS	Evans and Murshudov, 2013	http://www.ccp4.ac.uk
Phenix	Adams et al., 2010	https://hkl-xray.com/
Coot	Emsley et al., 2010	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
Staraniso Server	Global Phasing	https://staraniso.globalphasing.org/cgi-bin/staraniso.cgi
Pymol	Schrӧdinger	https://pymol.org/2/
PDBePISA	Krissinel and Henrick, 2007	http://www.ebi.ac.uk/pdbe/pisa/
TLSMD server	Painter and Merritt, 2006	http://skuld.bmsc.washington.edu/~tlsmd/
Scrubber 2.0	BioLogic Software	http://www.biologic.com.au
SednTerp	Dr. Thomas Laue	http://bitcwiki.sr.unh.edu/index.php/Main_Page
HeteroAnalysis	Cole et al., 2008	https://core.uconn.edu/auf
Multalin	Corpet, 1988	http://multalin.toulouse.inra.fr/multalin/
ESPript	Robert and Gouet, 2014	http://espript.ibcp.fr
WebLogo 3.0	Crooks et al., 2004	http://weblogo.threeplusone.com/
Leginon	Suloway et al., 2005, 2009	https://emg.nysbc.org/redmine/projects/leginon/wiki/Leginon_Homepage
MotionCor2	Zheng et al., 2017	https://emcore.ucsf.edu/ucsfmotioncor2
Appion-Protomo	Lander et al., 2009; Noble and Stagg, 2015; Winkler and Taylor, 2006	https://emg.nysbc.org/redmine/projects/appion/wiki/Appion_Home; http://www.electrontomography.org/
Tomo3D SIRT	Agulleiro and Fernandez, 2015	https://sites.google.com/site/3demimageprocessing/tomo3d
3dmod	Kremer et al., 1996	https://bio3d.colorado.edu/imod/
Fiji	Schindelin et al., 2012	https://imagej.net/Fiji