Figure 3. Blockade current histograms for α-synuclein. (A) wild type, (B) mutant A30P, (C) mutant E46K and (D) mutant A53T. The peptides (rPeptide) were dissolved in 10 mM TRIS-HCl, pH 7.4 at 1 mg/ml and 10 μL was added to the cis-side of the nanopore chamber which contained 1 M KCl, 10 mM HEPES, 1 mM EDTA, pH 7.8. The applied voltage was 100 mV.