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. 2020 Mar 9;9:e52286. doi: 10.7554/eLife.52286

Table 3. Dimensions and currents used for each milling pattern during automated sequential lamellae preparation.

Milling Pattern # Pattern Attributes
Milling pattern 1 and 2
(Rough Milling 1)
30 kV 700 pA
9 × 5 µm rectangle
Generates 2 µm thick lamella
Milling Pattern 3 and 4
(Rough Milling 2)
30 kV 300 pA
8 × 2 µm rectangle
Generates 1 µm thick lamella
Milling Pattern 5 and 6
(Rough Milling 3)
30 kV 100 pA
7.5 × 1 µm rectangle
Generates 500 nm thick lamella
Milling Pattern 7 and 8
(Lamella Polishing)
30 kV 50 pA
7 × 0.5 µm rectangle
Generates 300 nm thick lamella
Drift Correction Pattern
(Rough Milling and Polishing)
3 × 3 µm rectangle