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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Environ Pollut. 2020 Jan 7;259:113908. doi: 10.1016/j.envpol.2019.113908

Fig. 1. m6A level increases in arsenite-transformed human keratinocytes.

Fig. 1

a, Cell proliferation of HaCaT and HaCaT-T cells was measured by MTT assay. b, Percentage of clone formation was calculated by soft agar clone formation assay in HaCaT and HaCaT-T cells (down). Representative images of HaCaT and HaCaT-T cells in soft agar clone formation assay observed with a microscope at 400× magnification (up). c, Percentage of colony formation was tested by plate colony formation assay in HaCaT and HaCaT-T cells (down). Representative images of HaCaT and HaCaT-T cells in plate colony formation (up). d, Total m6A levels in HaCaT and HaCaT-T cells of 0, 15, 30, 50 passages were determined by EpiQuik m6A RNA Methylation Quantification Kit. e, f, RT-qPCR testing the mRNA levels (e) and western blotting examining the protein levels (f) of m6A regulating proteins (METTL3, METTL14, WTAP, KIAA1429, FTO, and YTHDF1) in HaCaT and HaCaT-T cells of 0, 15, 30, 50 passages are shown in heat maps. Representative images of immunoblot (f, left). For a-f, n = at least 3 biological replicates, error bars indicate mean ± SEM. The P-values were determined by two-tailed t-test. “*” indicates a significant difference compared to control group (P < 0.05).