Table 2.

Allergenic proteins identified in Quercus rubra pollen.

Spota	Protein	Organism	Functional category	Accession numberb	Exper. Mr/pIc	Theo. Mr/pId	PM/SCe	Scoref
1	ATP synthase subunit beta	Quercus suber	ATP biosynthetic process	POF09998.1	52.8/5.66	83.3/5.93	13/20%	556
1	ATP synthase subunit alpha	Oenothera biennis	ATP biosynthetic process	P05492.1	52.8/5.66	55.8/6.23	2/6%	90
1	Xyloseisomeraseisoform X1	Quercus suber	Carbohydrate metabolism	XP_023880138.1	52.8/5.66	57.8/5.65	3/7%	89
1	Enolase 1, chloroplastic-like	Quercus suber	Glycolytic process	XP_023876868.1	52.8/5.66	51.6/5.72	3/8%	81
2	ATP synthase subunit beta,	Quercus suber	ATP biosynthetic process	XP_023880780.1	52.4/5.85	59.6/5.80	10/31%	384
2	Xyloseisomeraseisoform X1	Quercus suber	Carbohydrate metabolism	XP_023880138.1	52.4/5.85	57.8/5.65	4/9%	233
2	ATP synthase subunit alpha	Oenothera biennis	ATP biosynthetic process	P05492.1	52.4/5.85	55.8/6.23	4/10%	194
2	Enolase 1, chloroplastic-like	Quercus suber	Glycolytic process	XP_023876868.1	52.4/5.85	51.6/5.72	4/13%	58
3	Enolase 1	Quercus suber	Glycolytic process	XP_023928354.1	53.2/6.04	48.2/5.64	9/31%	363
3	Xyloseisomeraseisoform X1	Quercus suber	Carbohydrate metabolism	XP_023880138.1	53.2/6.04	57.8/5.65	5/13%	347
3	ATP synthase subunit alpha	Oenothera biennis	ATP biosynthetic process	P05492.1	53.2/6.04	55.8/6.23	2/6%	174
3	Aldehyde dehydrogenase	Quercus suber	Detoxification	XP_023903360.1	53.2/6.04	54.2/5.71	3/8%	124
4	Enolase 1	Quercus suber	Glycolytic process	XP_023928354.1	50.9/6.15	48.2/5.64	10/33%	497
4	Xyloseisomeraseisoform X1	Quercus suber	Carbohydrate metabolism	XP_023880138.1	50.9/6.15	57.8/5.65	4/9%	252
4	Aldehyde dehydrogenase	Quercus suber	Detoxification	XP_023903360.1	50.9/6.15	54.2/5.71	3/8%	154
4	UTP--glucose-1-phosphate uridylyltransferase	Quercus suber	UDP-glucose metabolic process	XP_023870341.1	50.9/6.15	52.2/6.14	3/9%	148
4	Beta-xylosidase/alpha-l-arabinofuranosidase 1-like	Quercus suber	Carbohydrate metabolism	XP_023884910.1	50.9/6.15	90.1/5.97	3/5%	131

^aSpot numbers as indicated in Fig. 2.

^bAccession numbers according to NCBInrdatabse.

^cExperimental molecular mass (kDa) and pI.

^dTheoretical mass (kDa) and pI of identified proteins retrieved from NCBInr database.

^eNumber of peptides matched/protein sequence coverage.

^fMascot score reported after database search, score >47 indicate identity or extensive homology at p < 0.05