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. 2020 Mar 13;11:178. doi: 10.3389/fphys.2020.00178

FIGURE 4.

FIGURE 4

Silencing of augmenter of liver regeneration (ALR) inhibited ischemia–reperfusion (I/R)-induced mitophagy in HK-2 cells. (A,B) Representative images and quantification of immunofluorescence double-labeling LC3B and translocase of outer mitochondrial membrane 20 (TOMM20) in different groups. TOMM20-labeled mitochondria (red) and LC3-labeled autophagosome (green). Scale bar: 50 μm. the Pearson’s correlation columns were shown in bar graph format. Sixty cells from three independent experiments were analyzed. (C,D) Immunoblot analysis and quantification of ALR, LC3, P62, TOMM20, and translocase of inner mitochondrial membrane 23 (TIMM23). Data represent the means ± SD. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.