Skip to main content
. 2020 Mar 13;11:178. doi: 10.3389/fphys.2020.00178

FIGURE 5.

FIGURE 5

Silencing of augmenter of liver regeneration (ALR) inhibited the activation of PINK1/Parkin pathway in HK-2 cells during ischemia–reperfusion (I/R). (A–D) Representative images and quantification of immunofluorescence double-labeling PINK1/Parkin and mitochondrial marker [translocase of outer mitochondrial membrane 20 (TOMM20)]. PINK1 or Parkin was labeled with fluorescein isothiocyanate (FITC) (green), mitochondria was labeled with tetramethylrhodamine isothiocyanate (TRITC) (red). Scale bar = 50 μm. the Pearson’s correlation columns were shown in bar graph format. Sixty cells from three independent experiments were analyzed. (E,F) Immunoblot analysis and quantification of PINK1 and Parkin. Data represent means ± SD. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.