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. 2020 Mar 13;11:178. doi: 10.3389/fphys.2020.00178

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Copyright © 2020 Zhu, Liao, Huang, Sun, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of augmenter of liver regeneration (ALR) aggravated ischemia–reperfusion (I/R)-induced mitochondrial dysfunction in HK-2 cells. (A) Representative fluorescence images of HK-2 cells stained by JC-1. The picture was detected by confocal laser scanning microscopy (CLSM). Scale bar = 10 μm. (B,C) Representative images and analysis of mitochondrial membrane potential (MMP) via flow cytometry. (D) ATP contents. Using an ATP assay kit to measure ATP levels for each group. Data represent as the means ± SD. n = 3. **p < 0.01, ***p < 0.001, ****p < 0.0001.