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. 2020 Mar 13;11:263. doi: 10.3389/fphar.2020.00263

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Copyright © 2020 Grasso, Medeiros, Zampieri, Bol, Danhier, van Gisbergen, Bouzin, Brusa, Grégoire, Smeets, Stassen, Dubois, Lambin, Dutreix and Sonveaux.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial abundance and morphology differ between radiosensitive and radioresistant SQD9 cells. (A) Mitochondria were labeled with an anti-Tom20 antibody (green), the cytoskeleton with an anti-actin antibody (red) and cell nuclei with DAPI (blue). Representative pictures are shown on top (bar = 20 μm), and graphs show the number of mitochondrial networks per cell (left), the ratio of networked/individual mitochondria (middle) and the relative surface of cells occupied by mitochondria (MitoFootprint, right) (n = 20–34). (B) mtDNA content normalized to nuclear DNA content (n = 8). ^∗P < 0.05, ^∗∗∗P < 0.005 by Student’s t-test.