FIGURE 3.

Oxidative SQD9 are more radioresistant than glycolytic ones. (A) OCRs corresponding to basal respiration, maximal respiration and the oxidative contribution to ATP production (n = 16). (B) Glucose consumption (left) and lactate production (right) 72 h after cell seeding (n = 5–6). (C) Intracellular glucose and lactate concentrations normalized by total protein expression (n = 4–10). (D) Clonogenicity of cells irradiated at increasing doses of Ɣ-rays. The left graph shows surviving fractions, the middle graph LD₅₀ and the right graph the D₀ dose (n = 9). (E) In vivo SQD9-HGU and SQD9-LGU tumor growth is shown on the left graph, and tumor doubling times on the right graph. Tumors were not irradiated (n = 5–6). ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.005; ns P > 0.05 for the same parameter between SQD9-HGU and SQD9-LGU; ^###P < 0.005 versus basal respiration of the same cell line; by Student’s t-test (A–C,D middle, D right, E right); or two-way ANOVA followed by a Sidak’s multiple comparison test (D left).