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. 2020 Mar 13;11:263. doi: 10.3389/fphar.2020.00263

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Copyright © 2020 Grasso, Medeiros, Zampieri, Bol, Danhier, van Gisbergen, Bouzin, Brusa, Grégoire, Smeets, Stassen, Dubois, Lambin, Dutreix and Sonveaux.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing hexokinases in SQD9-HGU and SQD9-LGU cells. (A–E) SQD9-HGU and SQD-9-LGU cells were lipofected with a cocktail of siRNAs targeting either HK1 or HK2 or both together. (A) Representative western blots show the expression of HK1, HK2 and GAPDH, which is quantified in the graphs (n = 2). (B) Glucose consumption (left) and lactate production (right) 72 h after cell seeding (n = 2). (C) OCRs corresponding to basal respiration, maximal respiration and the oxidative contribution to ATP production (n = 3–5). (D) Cell number measured over time (n = 4). (E) Clonogenic assay after irradiation at the indicated doses, where the graphs show surviving fractions (n = 4). *P < 0.05, **P < 0.01, ***P < 0.005, ns P > 0.05 by student’s t-test (C) or by two-way ANOVA followed by a Sidak’s multiple comparison test (D,E).