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. 2020 Mar 13;11:395. doi: 10.3389/fmicb.2020.00395

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Copyright © 2020 Silvestri, Turina, Fiorilli, Miozzi, Venice, Bonfante and Lanfranco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of Gigaspora margarita(Gma)-sRNA generating loci. (A) Biplot of principal components 1 and 2 of PCA based on the length of loci, the total number of mapped reads and the nucleotide size proportion of Gma-sRNAs (from 18 to 35 nt) defining each locus (19 total variables). (B) HDBSCAN clustering reveals the presence of two distinct populations of data (Clusters 1 and 2). (C) Overview of the relative genomic positions of the loci compared to those of protein-encoding genes. (D) Overview of the homology of Gma-sRNA-generating loci with fungal repetitive elements in RepBase 23.04 (tblastx: E-value ≤ 0.00005).