Fig. 7.
Attenuated phosphorylation of MLC20 and MYPT1 in C2α- and C2β-depleted HASM. a Effects of C2α and C2β knockdown on the expression of smooth muscle-specific proteins in HASM. Cells were transfected with control-, C2α- and C2β-siRNAs and subjected to Western blot analyses. Left, Western blots. Middle and right panels, quantified data of Mhc11, MLC20, MLCK, MYPT1 and αSMA. b Attenuated mono- and di-phosphorylation of MLC20 and phosphorylation of MYPT1 in C2α- and C2β-double depleted HASM. HASM were transfected with control-, C2α- and C2β-siRNAs and stimulated with 0.3 µM ionomycin for 10 min. ★p < 0.05 compared with control siRNA-transfected cells. c Effects of ROCK inhibitor Y-27632 on mono- and di-phosphorylation of MLC20 and phosphorylation of MYPT1 in C2α- and C2β-double depleted HASM. ★p < 0.05, ★★p < 0.01 and ★★★p < 0.001 in the indicated comparisons